DETEKSI PEPPER VEIN YELLOWS VIRUS YANG BERASOSIASI DENGAN PENYAKIT YELLOW VEIN BANDING PADA TANAMAN MENTIMUN DI TABANAN - BALI
Abstract
Gejala menguning antara lamina daun (yellow vein banding) telah ditemukan pada tanaman mentimun di Bali sejak tahun 2014. Gejala pada tanaman mentimun tersebut memiliki kemiripan dengan gejala pada tanaman cabai yang disebabkan oleh infeksi Pepper vein yellow virus, anggota genus Polerovirus. Tujuan penelitian ialah membuktikan penyebab penyakit menguning antara lamina daun pada tanaman mentimun di Bali. Metode identifikasi menggunakan RT-PCR dan analisis sikuensing. Sepasang primer spesifik PeVYV-CP-F-BamH1/PeVYV-CP-R-Pst1 berhasil mengamplifikasi bagian protein selubung PeVYV dengan ukuran ± 650 pb. Analisis sikuen nukleotida menunjukkan bahwa isolat mentimun Bali memiliki kemiripan > 95% dengan isolat PeVYV asal Bali, dan Rembang (Indonesia), Jepang serta Yunani yang menginfeksi tanaman cabai.Kata kunci: Polerovirus, protein selubung, RT-PCR, sikuen nukleotida.References
Alabi OJ, Al Rwahnih M, Jifon JL, Gregg L, Crosby KM, Mirkov TE. 2015. First report of Pepper vein yellows virus infecting pepper (Capsicum spp.) in the United States. Plant Dis. 99(11):1656. DOI: http:// dx.doi.org/10.1094/PDIS-03-15-0329-PDN.
Alfaro-Fernández A, ElShafie EE, Ali MA, El Bashir OOA, Córdoba-Sellés MC, Font MI. 2014. First report of Pepper vein yellows virus infecting hot pepper in Sudan. Plant Dis. 98(10):1446. DOI: http://dx.doi. org/10.1094/PDIS-03-14-0251-PDN.
D’Arcy CJ, Domier LL. 2005. Family Luteoviridae. Di dalam: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA, editor. Virus Taxonomy, Eighth Report of The International Committee of Taxonomy of Viruses. San Diego (US): Elsevier Academic Pr. hlm 891–900.
Dombrovsky A, Glanz E, Pearlsman M, Lachman O, Doron-Faigenboim A, Antignus Y. 2013. The complete genomic sequence of Pepper yellow leaf curl virus (PYLCV) and its implications for our understanding of evolution dynamics in the genus Polerovirus. PLoS One. 8(7):1–11. DOI: http://dx.doi.org/10.1371/journal. pone.0070722.
Doyle JJ, Doyle JJ. 1987. A rapid DNA isolation of procedure for small quantities of fresh leaf tissue. Phytochem Bull. 19:11–19.
Ito T, Sharma P, Kittipakorn K, Ikegami M. 2008. Complete nucleotide sequence of a new isolate of Tomato leaf curl New Delhi virus infecting cucumber, bottle gourd, and muskmelon in Thailand. Arch. Virol. 153:611–613. DOI: http://dx.doi. org/10.1007/s00705-007-0029-y.
Knierim D, Tsai WS, Maiss E, Kenyon L. 2014. Molecular diversity of Poleroviruses infecting cucurbit crops in four countries reveals the presence of members of six distinct species. Arch Virol. 159(6):1459– 1465. DOI: http://dx.doi.org/10.1007/ s00705-013-1939-5.
Mizutani T, Daryono BS, Ikegami M, Natsuaki KT. 2011. First report of Tomato leaf curl New Delhi virus infecting cucumber in Central Java, Indonesia. Plant Dis. 95(11): 1485. DOI: http://dx.doi.org/10.1094/ PDIS-03-11-0196.
Murakami R, Nakashima N, Hinomoto N, Kawano S, Toyosato T. 2011. The genome sequence of pepper vein yellows virus (family Luteoviridae, genus Polerovirus). Arch Virol. 156:921–923. DOI: http:// dx.doi.org/10.1007/s00705-011-0956-5.
Septariani DN, Hidayat SH, Nurhayati E. 2014. Identifikasi penyebab penyakit daun keriting kuning pada tanaman mentimun. J HPT Tropika 14(1): 80–86.
Suastika G, Hartono S, Nyana IDN, Natsuaki T. 2012. Laporan pertama tentang infeksi Polerovirus pada tanaman cabai di Daerah Bali, Indonesia. J. Fitopatol Indones. 8(5):151–154.
Villanueva F, Castillo P, Font MI, Alfaro-Fernández A, Moriones E, Navas-Castillo J. 2013. First report of Pepper vein yellows virus infecting sweet pepper in Spain. Plant Dis. 97(9):1261. DOI: http://dx.doi. org/10.1094/PDIS-04-13-0369-PDN.
Wiratama IDMP, Wirya GNAS, Adnyani NNP, Nyana IDN, Suastika G. 2015. Laporan pertama infeksi Begomovirus pada tanaman mentimun di Bali. J Fitopatol Indones. 11(5):175–178. DOI: http:// dx.doi.org/10.14692/jfi.11.5.175.
Yonaha T, Toyosato T, Kawano S, Osaki T. 1995. Pepper vein yellows virus, a novel Luteovirus from bell pepper plants in Japan. Ann Phytopathol Soc Jpn. 61:178–DOI: http://dx.doi.org/10.3186/ jjphytopath.61.178
Zhang SB, Zhao ZB, Zhang DY, Liu Y, Luo XW, Liu J. 2015. First report of Pepper vein yellows virus infecting red pepper in Mainland China. Plant Dis. 99(8):1190. DOI: http://dx.doi.org/10.1094/PDIS-01-15-0025-PDN.
